Introduction After a long gestation we’ll be releasing a new version of FastQC in the near future to address some of the common problems and confusions we’ve encountered in the current version. I’ll write more about this in future posts but wanted to start with the most common complaint, that the duplicate sequence plot was…
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Interpreting the duplicate sequence plot in FastQC
Background The one analysis module which seems to elicit more questions than any other is the duplicate sequence plot. Of all of the plots which the program generates it’s probably the one which causes the most warnings / errors in otherwise nice looking data. I’m happy to admit that it’s not always immediately obvious what…